- MEN pathway Mutants
- tem1∆
- tem1∆ net1-ts
- tem1-ts GAL-CDC15
- tem1-ts multicopy CDC14
- cdc15∆
- cdc15-ts multicopy TEM1
- cdc15-ts multicopy CDC14
- cdc15∆ net1-ts
- cdc15∆ net1-ts cdh1∆
- TAB6-1 cdc15∆
- GAL-TEM1
- mad2∆ GAL-TEM1 in nocodazole
- Multi-copy CDC15
mad2∆ GAL-TEM1 in nocodazole
debug: ,
test user =
test db =
Simulation:
Change of parameters: kmad2=0.01, ksspn=0, TEM1T=20, MDT=150.
Arrest: Mitotic exit at t=218.4 min.
Experiments:
Alexandru, G., Zachariae, W., Schleiffer, A. and Nasmyth, K. (1999). Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage. EMBO J. 18:2707-2721.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Fig. 9B, Clb2 degradation occurs earlier than for
mad2∆ in nocodazole.
Comments: